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anti-engrailed 4d9 (1:5, dshb, ia) mouse primary antibody  (Developmental Studies Hybridoma Bank)


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    Structured Review

    Developmental Studies Hybridoma Bank anti-engrailed 4d9 (1:5, dshb, ia) mouse primary antibody
    Anti Engrailed 4d9 (1:5, Dshb, Ia) Mouse Primary Antibody, supplied by Developmental Studies Hybridoma Bank, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/mouse+anti-engrailed/monoclonal+anti+engrailed+antibody+4d9/bio_rxiv__2025__07__02__662832-340-16-19
    Average 90 stars, based on 1 article reviews
    anti-engrailed 4d9 (1:5, dshb, ia) mouse primary antibody - by Bioz Stars, 2026-09
    90/100 stars

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    Related Articles

    Concentration Assay:

    Article Title: Emerin preserves stem cell survival through maintenance of centrosome and nuclear lamina structure.
    Article Snippet: Primary antibodies included: 1) mouse anti-Engrailed at 1:50 (DSHB 4D9); 2) mouse anti-Spectrin at D ev el o pm en t • A cc ep te d m an us cr ip t 1:50 (DSHB 3A9); 3) rabbit anti-Vasa at 1:500 (provided by Zissimos Mourelatos); 4) mouse anti--Tubulin at 1:1,000 (Millipore DM1A); 5) mouse anti-Lamin B (LamB also known as Lamin Dm0) at 1:300 (DSHB ADL84.12); 6) rabbit anti-Plp at 1:1000 (provided by Greg Rogers); 7) rabbit anti-Centrosomin, Cnn at 1:10,000 (provided by Nassar Rusan); 8) rabbit antiphosphoserine10 Histone 3 at 1:1000 (Abcam 47297); 9) goat anti-GFP at 1:2000 (Abcam 6673); 10) rabbit anti-Asterless, Asl at 1:1000 (provided by Nassar Rusan).

    Article Title: Polarization of Myosin II Refines Tissue Material Properties to Buffer Mechanical Stress
    Article Snippet: Primary antibodies used were rat anti-E-cad (Developmental Studies Hybridoma Bank), rabbit anti-Diaphanous , mouse anti-Engrailed (Developmental Studies Hybridoma Bank), mouse anti-Sqh (gift from R. Ward) , rabbit anti-phospho-Sqh (3671, Cell Signalling) goat anti-GFP-FITC (Abcam, ab6662).

    Article Title: Tissue-Specific Regulation of Translational Readthrough Tunes Functions of the Traffic Jam Transcription Factor
    Article Snippet: Primary antibodies used were: guinea pig anti-Tj (1:10,000; a kind gift from Dorothea Godt, University of Toronto, Canada), rabbit anti-Vasa (1:5,000; a gift from Herbert Jäckle, MPI-bpc, Göttingen), mouse anti-Engrailed (1:20; Developmental Studies Hybridoma Bank, DSHB), mouse anti-Fas3 (1:20; DSHB), rat anti-DE-Cadherin (1:20; DSHB), rabbit anti-mCherry (1:200; Abcam), and mouse anti-Flag (1:500; Sigma Aldrich).

    Article Title: Hh signaling from de novo organizers drive lgl neoplasia in Drosophila epithelium
    Article Snippet: Primary antibodies used were: Mouse anti-Engrailed (1:50; DSHB (Developmental Studies Hybridoma Bank)), Rat anti-Ci (1:50; DSHB), mouse anti-Ptc (1:50, DSHB), Rabbit anti-pSmad (1:200; Cell Signaling Technology), Mouse anti-Wingless (Wg) (1:250; DSHB), Rabbit anti-Vg (1:100; gift from S Carroll); Mouse anti–β-gal (1:500; Sigma Aldrich), Rabbit anti-Caspase 3 (1:1000, Sigma Aldrich); Alexa Fluor 555, 633 secondary antibodies (1:500), Phalloidin-633 (1:100).

    Article Title: Lineage-specific determination of ring neuron circuitry in the central complex of Drosophila
    Article Snippet: Primary antibodies used were: mouse anti-Dachshund, 1:20 (mAbdac2-3, Developmental Studies Hybridoma Bank, DSHB); mouse anti-Engrailed, 1:2 (4D9, DSHB); rabbit and chicken anti-GFP, 1:500 (Thermo Fisher Scientific/Invitrogen, A6455 and Ab13970, Abcam, respectively); goat anti-HRP (Cy3 conjugated- 123-165-021, Cy5 conjugated–115-175-146), 1:50 (all Jackson ImmunoResearch Labs); rabbit anti-Poxn, 1:200 ( ; from M. Noll, University of Zurich); mouse anti-Poxn, 1:100 ( ; a kind gift from A. Ghysen, University of Montpellier); rabbit anti-Poxn, 1:400 (generated as described above); mouse anti-Synapsin, 1:50 (3C11, DSHB); mouse anti-Brp, 1:20 (nc82, DSHB); rabbit anti-Miranda, 1:200 ( ); mouse anti-Prospero, 1:5 (mAbMR1A, DSHB); rabbit anti-pH3, 1:400 (06-570, Sigma-Aldrich);.

    Article Title: Mask exhibits trxG-like behavior and associates with H3K27ac marked chromatin.
    Article Snippet: The Trithorax group (trxG) proteins counteract the repressive effect of Polycomb group (PcG) complexes and maintain transcriptional memory of active states of key developmental genes.. Although chromatin structure and modifications appear to play a fundamental role in this process, it is not clear how trxG prevents PcG-silencing and heritably maintains an active gene expression state.. Here, we report a hitherto unknown role of Drosophila Multiple ankyrin repeats single KH domain (Mask), which emerged as one of the candidate trxG genes in our reverse genetic screen.

    Article Title: Tissue-specific regulation of translational readthrough tunes functions of the traffic jam transcription factor
    Article Snippet: Primary antibodies used were: guinea pig anti-Tj (1:10 000; a kind gift from Dorothea Godt, University of Toronto, Canada), rabbit anti-Vasa (1:5000; a gift from Herbert Jäckle, MPI-bpc, Göttingen), mouse anti-Engrailed (1:20; Developmental Studies Hybridoma Bank, DSHB), mouse anti-Fas3 (1:20; DSHB), rat anti-DE-Cadherin (1:20; DSHB), rabbit anti-mCherry (1:200; Abcam), and mouse anti-Flag (1:500; Sigma Aldrich).

    Article Title: Spatio-temporal regulation of Dachsous proteins during Drosophila development
    Article Snippet: Primary antibodies used were: rabbit anti-β-galactosidase (1:10000; Cappel), mouse anti-Engrailed (1:50 Developmental Studies Hybridoma Bank, DSHB), rabbit anti-Ds (Ds ex 1:1000 a gift from D.Strutt ( ) and guinea pig anti-Ds (Ds cyt 1:500 ( ).

    Western Blot:

    Article Title: Emerin preserves stem cell survival through maintenance of centrosome and nuclear lamina structure.
    Article Snippet: Primary antibodies included: 1) mouse anti-Engrailed at 1:50 (DSHB 4D9); 2) mouse anti-Spectrin at D ev el o pm en t • A cc ep te d m an us cr ip t 1:50 (DSHB 3A9); 3) rabbit anti-Vasa at 1:500 (provided by Zissimos Mourelatos); 4) mouse anti--Tubulin at 1:1,000 (Millipore DM1A); 5) mouse anti-Lamin B (LamB also known as Lamin Dm0) at 1:300 (DSHB ADL84.12); 6) rabbit anti-Plp at 1:1000 (provided by Greg Rogers); 7) rabbit anti-Centrosomin, Cnn at 1:10,000 (provided by Nassar Rusan); 8) rabbit antiphosphoserine10 Histone 3 at 1:1000 (Abcam 47297); 9) goat anti-GFP at 1:2000 (Abcam 6673); 10) rabbit anti-Asterless, Asl at 1:1000 (provided by Nassar Rusan).

    Article Title: Polarization of Myosin II Refines Tissue Material Properties to Buffer Mechanical Stress
    Article Snippet: Primary antibodies used were rat anti-E-cad (Developmental Studies Hybridoma Bank), rabbit anti-Diaphanous , mouse anti-Engrailed (Developmental Studies Hybridoma Bank), mouse anti-Sqh (gift from R. Ward) , rabbit anti-phospho-Sqh (3671, Cell Signalling) goat anti-GFP-FITC (Abcam, ab6662).

    Article Title: Tissue-Specific Regulation of Translational Readthrough Tunes Functions of the Traffic Jam Transcription Factor
    Article Snippet: Primary antibodies used were: guinea pig anti-Tj (1:10,000; a kind gift from Dorothea Godt, University of Toronto, Canada), rabbit anti-Vasa (1:5,000; a gift from Herbert Jäckle, MPI-bpc, Göttingen), mouse anti-Engrailed (1:20; Developmental Studies Hybridoma Bank, DSHB), mouse anti-Fas3 (1:20; DSHB), rat anti-DE-Cadherin (1:20; DSHB), rabbit anti-mCherry (1:200; Abcam), and mouse anti-Flag (1:500; Sigma Aldrich).

    Article Title: Hh signaling from de novo organizers drive lgl neoplasia in Drosophila epithelium
    Article Snippet: Primary antibodies used were: Mouse anti-Engrailed (1:50; DSHB (Developmental Studies Hybridoma Bank)), Rat anti-Ci (1:50; DSHB), mouse anti-Ptc (1:50, DSHB), Rabbit anti-pSmad (1:200; Cell Signaling Technology), Mouse anti-Wingless (Wg) (1:250; DSHB), Rabbit anti-Vg (1:100; gift from S Carroll); Mouse anti–β-gal (1:500; Sigma Aldrich), Rabbit anti-Caspase 3 (1:1000, Sigma Aldrich); Alexa Fluor 555, 633 secondary antibodies (1:500), Phalloidin-633 (1:100).

    Article Title: Lineage-specific determination of ring neuron circuitry in the central complex of Drosophila
    Article Snippet: Primary antibodies used were: mouse anti-Dachshund, 1:20 (mAbdac2-3, Developmental Studies Hybridoma Bank, DSHB); mouse anti-Engrailed, 1:2 (4D9, DSHB); rabbit and chicken anti-GFP, 1:500 (Thermo Fisher Scientific/Invitrogen, A6455 and Ab13970, Abcam, respectively); goat anti-HRP (Cy3 conjugated- 123-165-021, Cy5 conjugated–115-175-146), 1:50 (all Jackson ImmunoResearch Labs); rabbit anti-Poxn, 1:200 ( ; from M. Noll, University of Zurich); mouse anti-Poxn, 1:100 ( ; a kind gift from A. Ghysen, University of Montpellier); rabbit anti-Poxn, 1:400 (generated as described above); mouse anti-Synapsin, 1:50 (3C11, DSHB); mouse anti-Brp, 1:20 (nc82, DSHB); rabbit anti-Miranda, 1:200 ( ); mouse anti-Prospero, 1:5 (mAbMR1A, DSHB); rabbit anti-pH3, 1:400 (06-570, Sigma-Aldrich);.

    Article Title: Mask exhibits trxG-like behavior and associates with H3K27ac marked chromatin.
    Article Snippet: The Trithorax group (trxG) proteins counteract the repressive effect of Polycomb group (PcG) complexes and maintain transcriptional memory of active states of key developmental genes.. Although chromatin structure and modifications appear to play a fundamental role in this process, it is not clear how trxG prevents PcG-silencing and heritably maintains an active gene expression state.. Here, we report a hitherto unknown role of Drosophila Multiple ankyrin repeats single KH domain (Mask), which emerged as one of the candidate trxG genes in our reverse genetic screen.

    Article Title: Tissue-specific regulation of translational readthrough tunes functions of the traffic jam transcription factor
    Article Snippet: Primary antibodies used were: guinea pig anti-Tj (1:10 000; a kind gift from Dorothea Godt, University of Toronto, Canada), rabbit anti-Vasa (1:5000; a gift from Herbert Jäckle, MPI-bpc, Göttingen), mouse anti-Engrailed (1:20; Developmental Studies Hybridoma Bank, DSHB), mouse anti-Fas3 (1:20; DSHB), rat anti-DE-Cadherin (1:20; DSHB), rabbit anti-mCherry (1:200; Abcam), and mouse anti-Flag (1:500; Sigma Aldrich).

    Article Title: Spatio-temporal regulation of Dachsous proteins during Drosophila development
    Article Snippet: Primary antibodies used were: rabbit anti-β-galactosidase (1:10000; Cappel), mouse anti-Engrailed (1:50 Developmental Studies Hybridoma Bank, DSHB), rabbit anti-Ds (Ds ex 1:1000 a gift from D.Strutt ( ) and guinea pig anti-Ds (Ds cyt 1:500 ( ).

    Immunofluorescence:

    Article Title: Emerin preserves stem cell survival through maintenance of centrosome and nuclear lamina structure.
    Article Snippet: Primary antibodies included: 1) mouse anti-Engrailed at 1:50 (DSHB 4D9); 2) mouse anti-Spectrin at D ev el o pm en t • A cc ep te d m an us cr ip t 1:50 (DSHB 3A9); 3) rabbit anti-Vasa at 1:500 (provided by Zissimos Mourelatos); 4) mouse anti--Tubulin at 1:1,000 (Millipore DM1A); 5) mouse anti-Lamin B (LamB also known as Lamin Dm0) at 1:300 (DSHB ADL84.12); 6) rabbit anti-Plp at 1:1000 (provided by Greg Rogers); 7) rabbit anti-Centrosomin, Cnn at 1:10,000 (provided by Nassar Rusan); 8) rabbit antiphosphoserine10 Histone 3 at 1:1000 (Abcam 47297); 9) goat anti-GFP at 1:2000 (Abcam 6673); 10) rabbit anti-Asterless, Asl at 1:1000 (provided by Nassar Rusan).

    Article Title: Polarization of Myosin II Refines Tissue Material Properties to Buffer Mechanical Stress
    Article Snippet: Primary antibodies used were rat anti-E-cad (Developmental Studies Hybridoma Bank), rabbit anti-Diaphanous , mouse anti-Engrailed (Developmental Studies Hybridoma Bank), mouse anti-Sqh (gift from R. Ward) , rabbit anti-phospho-Sqh (3671, Cell Signalling) goat anti-GFP-FITC (Abcam, ab6662).

    Article Title: Tissue-Specific Regulation of Translational Readthrough Tunes Functions of the Traffic Jam Transcription Factor
    Article Snippet: Primary antibodies used were: guinea pig anti-Tj (1:10,000; a kind gift from Dorothea Godt, University of Toronto, Canada), rabbit anti-Vasa (1:5,000; a gift from Herbert Jäckle, MPI-bpc, Göttingen), mouse anti-Engrailed (1:20; Developmental Studies Hybridoma Bank, DSHB), mouse anti-Fas3 (1:20; DSHB), rat anti-DE-Cadherin (1:20; DSHB), rabbit anti-mCherry (1:200; Abcam), and mouse anti-Flag (1:500; Sigma Aldrich).

    Article Title: Hh signaling from de novo organizers drive lgl neoplasia in Drosophila epithelium
    Article Snippet: Primary antibodies used were: Mouse anti-Engrailed (1:50; DSHB (Developmental Studies Hybridoma Bank)), Rat anti-Ci (1:50; DSHB), mouse anti-Ptc (1:50, DSHB), Rabbit anti-pSmad (1:200; Cell Signaling Technology), Mouse anti-Wingless (Wg) (1:250; DSHB), Rabbit anti-Vg (1:100; gift from S Carroll); Mouse anti–β-gal (1:500; Sigma Aldrich), Rabbit anti-Caspase 3 (1:1000, Sigma Aldrich); Alexa Fluor 555, 633 secondary antibodies (1:500), Phalloidin-633 (1:100).

    Article Title: Lineage-specific determination of ring neuron circuitry in the central complex of Drosophila
    Article Snippet: Primary antibodies used were: mouse anti-Dachshund, 1:20 (mAbdac2-3, Developmental Studies Hybridoma Bank, DSHB); mouse anti-Engrailed, 1:2 (4D9, DSHB); rabbit and chicken anti-GFP, 1:500 (Thermo Fisher Scientific/Invitrogen, A6455 and Ab13970, Abcam, respectively); goat anti-HRP (Cy3 conjugated- 123-165-021, Cy5 conjugated–115-175-146), 1:50 (all Jackson ImmunoResearch Labs); rabbit anti-Poxn, 1:200 ( ; from M. Noll, University of Zurich); mouse anti-Poxn, 1:100 ( ; a kind gift from A. Ghysen, University of Montpellier); rabbit anti-Poxn, 1:400 (generated as described above); mouse anti-Synapsin, 1:50 (3C11, DSHB); mouse anti-Brp, 1:20 (nc82, DSHB); rabbit anti-Miranda, 1:200 ( ); mouse anti-Prospero, 1:5 (mAbMR1A, DSHB); rabbit anti-pH3, 1:400 (06-570, Sigma-Aldrich);.

    Article Title: Mask exhibits trxG-like behavior and associates with H3K27ac marked chromatin.
    Article Snippet: The Trithorax group (trxG) proteins counteract the repressive effect of Polycomb group (PcG) complexes and maintain transcriptional memory of active states of key developmental genes.. Although chromatin structure and modifications appear to play a fundamental role in this process, it is not clear how trxG prevents PcG-silencing and heritably maintains an active gene expression state.. Here, we report a hitherto unknown role of Drosophila Multiple ankyrin repeats single KH domain (Mask), which emerged as one of the candidate trxG genes in our reverse genetic screen.

    Article Title: Tissue-specific regulation of translational readthrough tunes functions of the traffic jam transcription factor
    Article Snippet: Primary antibodies used were: guinea pig anti-Tj (1:10 000; a kind gift from Dorothea Godt, University of Toronto, Canada), rabbit anti-Vasa (1:5000; a gift from Herbert Jäckle, MPI-bpc, Göttingen), mouse anti-Engrailed (1:20; Developmental Studies Hybridoma Bank, DSHB), mouse anti-Fas3 (1:20; DSHB), rat anti-DE-Cadherin (1:20; DSHB), rabbit anti-mCherry (1:200; Abcam), and mouse anti-Flag (1:500; Sigma Aldrich).

    Article Title: Spatio-temporal regulation of Dachsous proteins during Drosophila development
    Article Snippet: Primary antibodies used were: rabbit anti-β-galactosidase (1:10000; Cappel), mouse anti-Engrailed (1:50 Developmental Studies Hybridoma Bank, DSHB), rabbit anti-Ds (Ds ex 1:1000 a gift from D.Strutt ( ) and guinea pig anti-Ds (Ds cyt 1:500 ( ).

    Chromatin Immunoprecipitation:

    Article Title: Emerin preserves stem cell survival through maintenance of centrosome and nuclear lamina structure.
    Article Snippet: Primary antibodies included: 1) mouse anti-Engrailed at 1:50 (DSHB 4D9); 2) mouse anti-Spectrin at D ev el o pm en t • A cc ep te d m an us cr ip t 1:50 (DSHB 3A9); 3) rabbit anti-Vasa at 1:500 (provided by Zissimos Mourelatos); 4) mouse anti--Tubulin at 1:1,000 (Millipore DM1A); 5) mouse anti-Lamin B (LamB also known as Lamin Dm0) at 1:300 (DSHB ADL84.12); 6) rabbit anti-Plp at 1:1000 (provided by Greg Rogers); 7) rabbit anti-Centrosomin, Cnn at 1:10,000 (provided by Nassar Rusan); 8) rabbit antiphosphoserine10 Histone 3 at 1:1000 (Abcam 47297); 9) goat anti-GFP at 1:2000 (Abcam 6673); 10) rabbit anti-Asterless, Asl at 1:1000 (provided by Nassar Rusan).

    Article Title: Polarization of Myosin II Refines Tissue Material Properties to Buffer Mechanical Stress
    Article Snippet: Primary antibodies used were rat anti-E-cad (Developmental Studies Hybridoma Bank), rabbit anti-Diaphanous , mouse anti-Engrailed (Developmental Studies Hybridoma Bank), mouse anti-Sqh (gift from R. Ward) , rabbit anti-phospho-Sqh (3671, Cell Signalling) goat anti-GFP-FITC (Abcam, ab6662).

    Article Title: Tissue-Specific Regulation of Translational Readthrough Tunes Functions of the Traffic Jam Transcription Factor
    Article Snippet: Primary antibodies used were: guinea pig anti-Tj (1:10,000; a kind gift from Dorothea Godt, University of Toronto, Canada), rabbit anti-Vasa (1:5,000; a gift from Herbert Jäckle, MPI-bpc, Göttingen), mouse anti-Engrailed (1:20; Developmental Studies Hybridoma Bank, DSHB), mouse anti-Fas3 (1:20; DSHB), rat anti-DE-Cadherin (1:20; DSHB), rabbit anti-mCherry (1:200; Abcam), and mouse anti-Flag (1:500; Sigma Aldrich).

    Article Title: Hh signaling from de novo organizers drive lgl neoplasia in Drosophila epithelium
    Article Snippet: Primary antibodies used were: Mouse anti-Engrailed (1:50; DSHB (Developmental Studies Hybridoma Bank)), Rat anti-Ci (1:50; DSHB), mouse anti-Ptc (1:50, DSHB), Rabbit anti-pSmad (1:200; Cell Signaling Technology), Mouse anti-Wingless (Wg) (1:250; DSHB), Rabbit anti-Vg (1:100; gift from S Carroll); Mouse anti–β-gal (1:500; Sigma Aldrich), Rabbit anti-Caspase 3 (1:1000, Sigma Aldrich); Alexa Fluor 555, 633 secondary antibodies (1:500), Phalloidin-633 (1:100).

    Article Title: Lineage-specific determination of ring neuron circuitry in the central complex of Drosophila
    Article Snippet: Primary antibodies used were: mouse anti-Dachshund, 1:20 (mAbdac2-3, Developmental Studies Hybridoma Bank, DSHB); mouse anti-Engrailed, 1:2 (4D9, DSHB); rabbit and chicken anti-GFP, 1:500 (Thermo Fisher Scientific/Invitrogen, A6455 and Ab13970, Abcam, respectively); goat anti-HRP (Cy3 conjugated- 123-165-021, Cy5 conjugated–115-175-146), 1:50 (all Jackson ImmunoResearch Labs); rabbit anti-Poxn, 1:200 ( ; from M. Noll, University of Zurich); mouse anti-Poxn, 1:100 ( ; a kind gift from A. Ghysen, University of Montpellier); rabbit anti-Poxn, 1:400 (generated as described above); mouse anti-Synapsin, 1:50 (3C11, DSHB); mouse anti-Brp, 1:20 (nc82, DSHB); rabbit anti-Miranda, 1:200 ( ); mouse anti-Prospero, 1:5 (mAbMR1A, DSHB); rabbit anti-pH3, 1:400 (06-570, Sigma-Aldrich);.

    Article Title: Mask exhibits trxG-like behavior and associates with H3K27ac marked chromatin.
    Article Snippet: The Trithorax group (trxG) proteins counteract the repressive effect of Polycomb group (PcG) complexes and maintain transcriptional memory of active states of key developmental genes.. Although chromatin structure and modifications appear to play a fundamental role in this process, it is not clear how trxG prevents PcG-silencing and heritably maintains an active gene expression state.. Here, we report a hitherto unknown role of Drosophila Multiple ankyrin repeats single KH domain (Mask), which emerged as one of the candidate trxG genes in our reverse genetic screen.

    Article Title: Tissue-specific regulation of translational readthrough tunes functions of the traffic jam transcription factor
    Article Snippet: Primary antibodies used were: guinea pig anti-Tj (1:10 000; a kind gift from Dorothea Godt, University of Toronto, Canada), rabbit anti-Vasa (1:5000; a gift from Herbert Jäckle, MPI-bpc, Göttingen), mouse anti-Engrailed (1:20; Developmental Studies Hybridoma Bank, DSHB), mouse anti-Fas3 (1:20; DSHB), rat anti-DE-Cadherin (1:20; DSHB), rabbit anti-mCherry (1:200; Abcam), and mouse anti-Flag (1:500; Sigma Aldrich).

    Article Title: Spatio-temporal regulation of Dachsous proteins during Drosophila development
    Article Snippet: Primary antibodies used were: rabbit anti-β-galactosidase (1:10000; Cappel), mouse anti-Engrailed (1:50 Developmental Studies Hybridoma Bank, DSHB), rabbit anti-Ds (Ds ex 1:1000 a gift from D.Strutt ( ) and guinea pig anti-Ds (Ds cyt 1:500 ( ).

    Recombinant:

    Article Title: Emerin preserves stem cell survival through maintenance of centrosome and nuclear lamina structure.
    Article Snippet: Primary antibodies included: 1) mouse anti-Engrailed at 1:50 (DSHB 4D9); 2) mouse anti-Spectrin at D ev el o pm en t • A cc ep te d m an us cr ip t 1:50 (DSHB 3A9); 3) rabbit anti-Vasa at 1:500 (provided by Zissimos Mourelatos); 4) mouse anti--Tubulin at 1:1,000 (Millipore DM1A); 5) mouse anti-Lamin B (LamB also known as Lamin Dm0) at 1:300 (DSHB ADL84.12); 6) rabbit anti-Plp at 1:1000 (provided by Greg Rogers); 7) rabbit anti-Centrosomin, Cnn at 1:10,000 (provided by Nassar Rusan); 8) rabbit antiphosphoserine10 Histone 3 at 1:1000 (Abcam 47297); 9) goat anti-GFP at 1:2000 (Abcam 6673); 10) rabbit anti-Asterless, Asl at 1:1000 (provided by Nassar Rusan).

    Article Title: Polarization of Myosin II Refines Tissue Material Properties to Buffer Mechanical Stress
    Article Snippet: Primary antibodies used were rat anti-E-cad (Developmental Studies Hybridoma Bank), rabbit anti-Diaphanous , mouse anti-Engrailed (Developmental Studies Hybridoma Bank), mouse anti-Sqh (gift from R. Ward) , rabbit anti-phospho-Sqh (3671, Cell Signalling) goat anti-GFP-FITC (Abcam, ab6662).

    Article Title: Tissue-Specific Regulation of Translational Readthrough Tunes Functions of the Traffic Jam Transcription Factor
    Article Snippet: Primary antibodies used were: guinea pig anti-Tj (1:10,000; a kind gift from Dorothea Godt, University of Toronto, Canada), rabbit anti-Vasa (1:5,000; a gift from Herbert Jäckle, MPI-bpc, Göttingen), mouse anti-Engrailed (1:20; Developmental Studies Hybridoma Bank, DSHB), mouse anti-Fas3 (1:20; DSHB), rat anti-DE-Cadherin (1:20; DSHB), rabbit anti-mCherry (1:200; Abcam), and mouse anti-Flag (1:500; Sigma Aldrich).

    Article Title: Hh signaling from de novo organizers drive lgl neoplasia in Drosophila epithelium
    Article Snippet: Primary antibodies used were: Mouse anti-Engrailed (1:50; DSHB (Developmental Studies Hybridoma Bank)), Rat anti-Ci (1:50; DSHB), mouse anti-Ptc (1:50, DSHB), Rabbit anti-pSmad (1:200; Cell Signaling Technology), Mouse anti-Wingless (Wg) (1:250; DSHB), Rabbit anti-Vg (1:100; gift from S Carroll); Mouse anti–β-gal (1:500; Sigma Aldrich), Rabbit anti-Caspase 3 (1:1000, Sigma Aldrich); Alexa Fluor 555, 633 secondary antibodies (1:500), Phalloidin-633 (1:100).

    Article Title: Lineage-specific determination of ring neuron circuitry in the central complex of Drosophila
    Article Snippet: Primary antibodies used were: mouse anti-Dachshund, 1:20 (mAbdac2-3, Developmental Studies Hybridoma Bank, DSHB); mouse anti-Engrailed, 1:2 (4D9, DSHB); rabbit and chicken anti-GFP, 1:500 (Thermo Fisher Scientific/Invitrogen, A6455 and Ab13970, Abcam, respectively); goat anti-HRP (Cy3 conjugated- 123-165-021, Cy5 conjugated–115-175-146), 1:50 (all Jackson ImmunoResearch Labs); rabbit anti-Poxn, 1:200 ( ; from M. Noll, University of Zurich); mouse anti-Poxn, 1:100 ( ; a kind gift from A. Ghysen, University of Montpellier); rabbit anti-Poxn, 1:400 (generated as described above); mouse anti-Synapsin, 1:50 (3C11, DSHB); mouse anti-Brp, 1:20 (nc82, DSHB); rabbit anti-Miranda, 1:200 ( ); mouse anti-Prospero, 1:5 (mAbMR1A, DSHB); rabbit anti-pH3, 1:400 (06-570, Sigma-Aldrich);.

    Article Title: Mask exhibits trxG-like behavior and associates with H3K27ac marked chromatin.
    Article Snippet: The Trithorax group (trxG) proteins counteract the repressive effect of Polycomb group (PcG) complexes and maintain transcriptional memory of active states of key developmental genes.. Although chromatin structure and modifications appear to play a fundamental role in this process, it is not clear how trxG prevents PcG-silencing and heritably maintains an active gene expression state.. Here, we report a hitherto unknown role of Drosophila Multiple ankyrin repeats single KH domain (Mask), which emerged as one of the candidate trxG genes in our reverse genetic screen.

    Article Title: Tissue-specific regulation of translational readthrough tunes functions of the traffic jam transcription factor
    Article Snippet: Primary antibodies used were: guinea pig anti-Tj (1:10 000; a kind gift from Dorothea Godt, University of Toronto, Canada), rabbit anti-Vasa (1:5000; a gift from Herbert Jäckle, MPI-bpc, Göttingen), mouse anti-Engrailed (1:20; Developmental Studies Hybridoma Bank, DSHB), mouse anti-Fas3 (1:20; DSHB), rat anti-DE-Cadherin (1:20; DSHB), rabbit anti-mCherry (1:200; Abcam), and mouse anti-Flag (1:500; Sigma Aldrich).

    Article Title: Spatio-temporal regulation of Dachsous proteins during Drosophila development
    Article Snippet: Primary antibodies used were: rabbit anti-β-galactosidase (1:10000; Cappel), mouse anti-Engrailed (1:50 Developmental Studies Hybridoma Bank, DSHB), rabbit anti-Ds (Ds ex 1:1000 a gift from D.Strutt ( ) and guinea pig anti-Ds (Ds cyt 1:500 ( ).

    Solvent:

    Article Title: Emerin preserves stem cell survival through maintenance of centrosome and nuclear lamina structure.
    Article Snippet: Primary antibodies included: 1) mouse anti-Engrailed at 1:50 (DSHB 4D9); 2) mouse anti-Spectrin at D ev el o pm en t • A cc ep te d m an us cr ip t 1:50 (DSHB 3A9); 3) rabbit anti-Vasa at 1:500 (provided by Zissimos Mourelatos); 4) mouse anti--Tubulin at 1:1,000 (Millipore DM1A); 5) mouse anti-Lamin B (LamB also known as Lamin Dm0) at 1:300 (DSHB ADL84.12); 6) rabbit anti-Plp at 1:1000 (provided by Greg Rogers); 7) rabbit anti-Centrosomin, Cnn at 1:10,000 (provided by Nassar Rusan); 8) rabbit antiphosphoserine10 Histone 3 at 1:1000 (Abcam 47297); 9) goat anti-GFP at 1:2000 (Abcam 6673); 10) rabbit anti-Asterless, Asl at 1:1000 (provided by Nassar Rusan).

    Article Title: Polarization of Myosin II Refines Tissue Material Properties to Buffer Mechanical Stress
    Article Snippet: Primary antibodies used were rat anti-E-cad (Developmental Studies Hybridoma Bank), rabbit anti-Diaphanous , mouse anti-Engrailed (Developmental Studies Hybridoma Bank), mouse anti-Sqh (gift from R. Ward) , rabbit anti-phospho-Sqh (3671, Cell Signalling) goat anti-GFP-FITC (Abcam, ab6662).

    Article Title: Tissue-Specific Regulation of Translational Readthrough Tunes Functions of the Traffic Jam Transcription Factor
    Article Snippet: Primary antibodies used were: guinea pig anti-Tj (1:10,000; a kind gift from Dorothea Godt, University of Toronto, Canada), rabbit anti-Vasa (1:5,000; a gift from Herbert Jäckle, MPI-bpc, Göttingen), mouse anti-Engrailed (1:20; Developmental Studies Hybridoma Bank, DSHB), mouse anti-Fas3 (1:20; DSHB), rat anti-DE-Cadherin (1:20; DSHB), rabbit anti-mCherry (1:200; Abcam), and mouse anti-Flag (1:500; Sigma Aldrich).

    Article Title: Hh signaling from de novo organizers drive lgl neoplasia in Drosophila epithelium
    Article Snippet: Primary antibodies used were: Mouse anti-Engrailed (1:50; DSHB (Developmental Studies Hybridoma Bank)), Rat anti-Ci (1:50; DSHB), mouse anti-Ptc (1:50, DSHB), Rabbit anti-pSmad (1:200; Cell Signaling Technology), Mouse anti-Wingless (Wg) (1:250; DSHB), Rabbit anti-Vg (1:100; gift from S Carroll); Mouse anti–β-gal (1:500; Sigma Aldrich), Rabbit anti-Caspase 3 (1:1000, Sigma Aldrich); Alexa Fluor 555, 633 secondary antibodies (1:500), Phalloidin-633 (1:100).

    Article Title: Lineage-specific determination of ring neuron circuitry in the central complex of Drosophila
    Article Snippet: Primary antibodies used were: mouse anti-Dachshund, 1:20 (mAbdac2-3, Developmental Studies Hybridoma Bank, DSHB); mouse anti-Engrailed, 1:2 (4D9, DSHB); rabbit and chicken anti-GFP, 1:500 (Thermo Fisher Scientific/Invitrogen, A6455 and Ab13970, Abcam, respectively); goat anti-HRP (Cy3 conjugated- 123-165-021, Cy5 conjugated–115-175-146), 1:50 (all Jackson ImmunoResearch Labs); rabbit anti-Poxn, 1:200 ( ; from M. Noll, University of Zurich); mouse anti-Poxn, 1:100 ( ; a kind gift from A. Ghysen, University of Montpellier); rabbit anti-Poxn, 1:400 (generated as described above); mouse anti-Synapsin, 1:50 (3C11, DSHB); mouse anti-Brp, 1:20 (nc82, DSHB); rabbit anti-Miranda, 1:200 ( ); mouse anti-Prospero, 1:5 (mAbMR1A, DSHB); rabbit anti-pH3, 1:400 (06-570, Sigma-Aldrich);.

    Article Title: Mask exhibits trxG-like behavior and associates with H3K27ac marked chromatin.
    Article Snippet: The Trithorax group (trxG) proteins counteract the repressive effect of Polycomb group (PcG) complexes and maintain transcriptional memory of active states of key developmental genes.. Although chromatin structure and modifications appear to play a fundamental role in this process, it is not clear how trxG prevents PcG-silencing and heritably maintains an active gene expression state.. Here, we report a hitherto unknown role of Drosophila Multiple ankyrin repeats single KH domain (Mask), which emerged as one of the candidate trxG genes in our reverse genetic screen.

    Article Title: Tissue-specific regulation of translational readthrough tunes functions of the traffic jam transcription factor
    Article Snippet: Primary antibodies used were: guinea pig anti-Tj (1:10 000; a kind gift from Dorothea Godt, University of Toronto, Canada), rabbit anti-Vasa (1:5000; a gift from Herbert Jäckle, MPI-bpc, Göttingen), mouse anti-Engrailed (1:20; Developmental Studies Hybridoma Bank, DSHB), mouse anti-Fas3 (1:20; DSHB), rat anti-DE-Cadherin (1:20; DSHB), rabbit anti-mCherry (1:200; Abcam), and mouse anti-Flag (1:500; Sigma Aldrich).

    Article Title: Spatio-temporal regulation of Dachsous proteins during Drosophila development
    Article Snippet: Primary antibodies used were: rabbit anti-β-galactosidase (1:10000; Cappel), mouse anti-Engrailed (1:50 Developmental Studies Hybridoma Bank, DSHB), rabbit anti-Ds (Ds ex 1:1000 a gift from D.Strutt ( ) and guinea pig anti-Ds (Ds cyt 1:500 ( ).

    Knock-In:

    Article Title: Emerin preserves stem cell survival through maintenance of centrosome and nuclear lamina structure.
    Article Snippet: Primary antibodies included: 1) mouse anti-Engrailed at 1:50 (DSHB 4D9); 2) mouse anti-Spectrin at D ev el o pm en t • A cc ep te d m an us cr ip t 1:50 (DSHB 3A9); 3) rabbit anti-Vasa at 1:500 (provided by Zissimos Mourelatos); 4) mouse anti--Tubulin at 1:1,000 (Millipore DM1A); 5) mouse anti-Lamin B (LamB also known as Lamin Dm0) at 1:300 (DSHB ADL84.12); 6) rabbit anti-Plp at 1:1000 (provided by Greg Rogers); 7) rabbit anti-Centrosomin, Cnn at 1:10,000 (provided by Nassar Rusan); 8) rabbit antiphosphoserine10 Histone 3 at 1:1000 (Abcam 47297); 9) goat anti-GFP at 1:2000 (Abcam 6673); 10) rabbit anti-Asterless, Asl at 1:1000 (provided by Nassar Rusan).

    Article Title: Polarization of Myosin II Refines Tissue Material Properties to Buffer Mechanical Stress
    Article Snippet: Primary antibodies used were rat anti-E-cad (Developmental Studies Hybridoma Bank), rabbit anti-Diaphanous , mouse anti-Engrailed (Developmental Studies Hybridoma Bank), mouse anti-Sqh (gift from R. Ward) , rabbit anti-phospho-Sqh (3671, Cell Signalling) goat anti-GFP-FITC (Abcam, ab6662).

    Article Title: Tissue-Specific Regulation of Translational Readthrough Tunes Functions of the Traffic Jam Transcription Factor
    Article Snippet: Primary antibodies used were: guinea pig anti-Tj (1:10,000; a kind gift from Dorothea Godt, University of Toronto, Canada), rabbit anti-Vasa (1:5,000; a gift from Herbert Jäckle, MPI-bpc, Göttingen), mouse anti-Engrailed (1:20; Developmental Studies Hybridoma Bank, DSHB), mouse anti-Fas3 (1:20; DSHB), rat anti-DE-Cadherin (1:20; DSHB), rabbit anti-mCherry (1:200; Abcam), and mouse anti-Flag (1:500; Sigma Aldrich).

    Article Title: Hh signaling from de novo organizers drive lgl neoplasia in Drosophila epithelium
    Article Snippet: Primary antibodies used were: Mouse anti-Engrailed (1:50; DSHB (Developmental Studies Hybridoma Bank)), Rat anti-Ci (1:50; DSHB), mouse anti-Ptc (1:50, DSHB), Rabbit anti-pSmad (1:200; Cell Signaling Technology), Mouse anti-Wingless (Wg) (1:250; DSHB), Rabbit anti-Vg (1:100; gift from S Carroll); Mouse anti–β-gal (1:500; Sigma Aldrich), Rabbit anti-Caspase 3 (1:1000, Sigma Aldrich); Alexa Fluor 555, 633 secondary antibodies (1:500), Phalloidin-633 (1:100).

    Article Title: Lineage-specific determination of ring neuron circuitry in the central complex of Drosophila
    Article Snippet: Primary antibodies used were: mouse anti-Dachshund, 1:20 (mAbdac2-3, Developmental Studies Hybridoma Bank, DSHB); mouse anti-Engrailed, 1:2 (4D9, DSHB); rabbit and chicken anti-GFP, 1:500 (Thermo Fisher Scientific/Invitrogen, A6455 and Ab13970, Abcam, respectively); goat anti-HRP (Cy3 conjugated- 123-165-021, Cy5 conjugated–115-175-146), 1:50 (all Jackson ImmunoResearch Labs); rabbit anti-Poxn, 1:200 ( ; from M. Noll, University of Zurich); mouse anti-Poxn, 1:100 ( ; a kind gift from A. Ghysen, University of Montpellier); rabbit anti-Poxn, 1:400 (generated as described above); mouse anti-Synapsin, 1:50 (3C11, DSHB); mouse anti-Brp, 1:20 (nc82, DSHB); rabbit anti-Miranda, 1:200 ( ); mouse anti-Prospero, 1:5 (mAbMR1A, DSHB); rabbit anti-pH3, 1:400 (06-570, Sigma-Aldrich);.

    Article Title: Mask exhibits trxG-like behavior and associates with H3K27ac marked chromatin.
    Article Snippet: The Trithorax group (trxG) proteins counteract the repressive effect of Polycomb group (PcG) complexes and maintain transcriptional memory of active states of key developmental genes.. Although chromatin structure and modifications appear to play a fundamental role in this process, it is not clear how trxG prevents PcG-silencing and heritably maintains an active gene expression state.. Here, we report a hitherto unknown role of Drosophila Multiple ankyrin repeats single KH domain (Mask), which emerged as one of the candidate trxG genes in our reverse genetic screen.

    Article Title: Tissue-specific regulation of translational readthrough tunes functions of the traffic jam transcription factor
    Article Snippet: Primary antibodies used were: guinea pig anti-Tj (1:10 000; a kind gift from Dorothea Godt, University of Toronto, Canada), rabbit anti-Vasa (1:5000; a gift from Herbert Jäckle, MPI-bpc, Göttingen), mouse anti-Engrailed (1:20; Developmental Studies Hybridoma Bank, DSHB), mouse anti-Fas3 (1:20; DSHB), rat anti-DE-Cadherin (1:20; DSHB), rabbit anti-mCherry (1:200; Abcam), and mouse anti-Flag (1:500; Sigma Aldrich).

    Article Title: Spatio-temporal regulation of Dachsous proteins during Drosophila development
    Article Snippet: Primary antibodies used were: rabbit anti-β-galactosidase (1:10000; Cappel), mouse anti-Engrailed (1:50 Developmental Studies Hybridoma Bank, DSHB), rabbit anti-Ds (Ds ex 1:1000 a gift from D.Strutt ( ) and guinea pig anti-Ds (Ds cyt 1:500 ( ).

    Software:

    Article Title: Emerin preserves stem cell survival through maintenance of centrosome and nuclear lamina structure.
    Article Snippet: Primary antibodies included: 1) mouse anti-Engrailed at 1:50 (DSHB 4D9); 2) mouse anti-Spectrin at D ev el o pm en t • A cc ep te d m an us cr ip t 1:50 (DSHB 3A9); 3) rabbit anti-Vasa at 1:500 (provided by Zissimos Mourelatos); 4) mouse anti--Tubulin at 1:1,000 (Millipore DM1A); 5) mouse anti-Lamin B (LamB also known as Lamin Dm0) at 1:300 (DSHB ADL84.12); 6) rabbit anti-Plp at 1:1000 (provided by Greg Rogers); 7) rabbit anti-Centrosomin, Cnn at 1:10,000 (provided by Nassar Rusan); 8) rabbit antiphosphoserine10 Histone 3 at 1:1000 (Abcam 47297); 9) goat anti-GFP at 1:2000 (Abcam 6673); 10) rabbit anti-Asterless, Asl at 1:1000 (provided by Nassar Rusan).

    Article Title: Polarization of Myosin II Refines Tissue Material Properties to Buffer Mechanical Stress
    Article Snippet: Primary antibodies used were rat anti-E-cad (Developmental Studies Hybridoma Bank), rabbit anti-Diaphanous , mouse anti-Engrailed (Developmental Studies Hybridoma Bank), mouse anti-Sqh (gift from R. Ward) , rabbit anti-phospho-Sqh (3671, Cell Signalling) goat anti-GFP-FITC (Abcam, ab6662).

    Article Title: Tissue-Specific Regulation of Translational Readthrough Tunes Functions of the Traffic Jam Transcription Factor
    Article Snippet: Primary antibodies used were: guinea pig anti-Tj (1:10,000; a kind gift from Dorothea Godt, University of Toronto, Canada), rabbit anti-Vasa (1:5,000; a gift from Herbert Jäckle, MPI-bpc, Göttingen), mouse anti-Engrailed (1:20; Developmental Studies Hybridoma Bank, DSHB), mouse anti-Fas3 (1:20; DSHB), rat anti-DE-Cadherin (1:20; DSHB), rabbit anti-mCherry (1:200; Abcam), and mouse anti-Flag (1:500; Sigma Aldrich).

    Article Title: Hh signaling from de novo organizers drive lgl neoplasia in Drosophila epithelium
    Article Snippet: Primary antibodies used were: Mouse anti-Engrailed (1:50; DSHB (Developmental Studies Hybridoma Bank)), Rat anti-Ci (1:50; DSHB), mouse anti-Ptc (1:50, DSHB), Rabbit anti-pSmad (1:200; Cell Signaling Technology), Mouse anti-Wingless (Wg) (1:250; DSHB), Rabbit anti-Vg (1:100; gift from S Carroll); Mouse anti–β-gal (1:500; Sigma Aldrich), Rabbit anti-Caspase 3 (1:1000, Sigma Aldrich); Alexa Fluor 555, 633 secondary antibodies (1:500), Phalloidin-633 (1:100).

    Article Title: Lineage-specific determination of ring neuron circuitry in the central complex of Drosophila
    Article Snippet: Primary antibodies used were: mouse anti-Dachshund, 1:20 (mAbdac2-3, Developmental Studies Hybridoma Bank, DSHB); mouse anti-Engrailed, 1:2 (4D9, DSHB); rabbit and chicken anti-GFP, 1:500 (Thermo Fisher Scientific/Invitrogen, A6455 and Ab13970, Abcam, respectively); goat anti-HRP (Cy3 conjugated- 123-165-021, Cy5 conjugated–115-175-146), 1:50 (all Jackson ImmunoResearch Labs); rabbit anti-Poxn, 1:200 ( ; from M. Noll, University of Zurich); mouse anti-Poxn, 1:100 ( ; a kind gift from A. Ghysen, University of Montpellier); rabbit anti-Poxn, 1:400 (generated as described above); mouse anti-Synapsin, 1:50 (3C11, DSHB); mouse anti-Brp, 1:20 (nc82, DSHB); rabbit anti-Miranda, 1:200 ( ); mouse anti-Prospero, 1:5 (mAbMR1A, DSHB); rabbit anti-pH3, 1:400 (06-570, Sigma-Aldrich);.

    Article Title: Mask exhibits trxG-like behavior and associates with H3K27ac marked chromatin.
    Article Snippet: The Trithorax group (trxG) proteins counteract the repressive effect of Polycomb group (PcG) complexes and maintain transcriptional memory of active states of key developmental genes.. Although chromatin structure and modifications appear to play a fundamental role in this process, it is not clear how trxG prevents PcG-silencing and heritably maintains an active gene expression state.. Here, we report a hitherto unknown role of Drosophila Multiple ankyrin repeats single KH domain (Mask), which emerged as one of the candidate trxG genes in our reverse genetic screen.

    Article Title: Tissue-specific regulation of translational readthrough tunes functions of the traffic jam transcription factor
    Article Snippet: Primary antibodies used were: guinea pig anti-Tj (1:10 000; a kind gift from Dorothea Godt, University of Toronto, Canada), rabbit anti-Vasa (1:5000; a gift from Herbert Jäckle, MPI-bpc, Göttingen), mouse anti-Engrailed (1:20; Developmental Studies Hybridoma Bank, DSHB), mouse anti-Fas3 (1:20; DSHB), rat anti-DE-Cadherin (1:20; DSHB), rabbit anti-mCherry (1:200; Abcam), and mouse anti-Flag (1:500; Sigma Aldrich).

    Article Title: Spatio-temporal regulation of Dachsous proteins during Drosophila development
    Article Snippet: Primary antibodies used were: rabbit anti-β-galactosidase (1:10000; Cappel), mouse anti-Engrailed (1:50 Developmental Studies Hybridoma Bank, DSHB), rabbit anti-Ds (Ds ex 1:1000 a gift from D.Strutt ( ) and guinea pig anti-Ds (Ds cyt 1:500 ( ).



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